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A Raman scattering study of the interactions of DNA with its water of hydration |
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| Authors: | Scott A. Lee Nong-Jian Tao Allan Rupprecht |
| Affiliation: | 1. Department of Physics &2. Astronomy, University of Toledo, M.S. #111, Toledo, OH, 43606, USA.Scott.Lee@utoledo.edu;4. Center for Bioelectronics and Biosensors, Biodesign Institute, School of Electrical, Computer, and Energy Engineering, Arizona State University, Tempe, AZ, 85287, USA.;5. Division of Physical Chemistry, Arrhenius Laboratory, University of Stockholm, S-106 91, Stockholm, Sweden. |
| Abstract: | Raman spectroscopy is used to probe the nature of the hydrogen bonds which hold the water of hydration to DNA. The ～ 3450?cm?1 molecular O–H stretching mode shows that the first six water molecules per base pair of the primary hydration shell are very strongly bound to the DNA. The observed shift in the peak position of this mode permits a determination of the length of the hydrogen bonds for these water molecules. These hydrogen bonds appear to be about 0.3?Å shorter than the hydrogen bonds in bulk water. The linewidth of this mode shows no significant changes above water contents of about 15 water molecules per base pair. This technique of using a vibrational spectroscopy to obtain structural information about the hydration shells of DNA could be used to study the hydration shells of other biomolecules. |
| Keywords: | DNA water of hydration hydrogen bonding |