The DNA Sequence-dependence of Nucleosome Positioning in vivo and in vitro |
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| Authors: | Andrew Travers Edwige Hiriart Mark Churcher Micaela Caserta Ernesto Di Mauro |
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| Institution: | 1. MRC Laboratory of Molecular Biology , Hills Road, Cambridge , CB2 0QH , UK;2. Fondation Pierre-Gilles de Gennes pour la Recherche , c/o LBPA, école Normale Supérieure de Cachan, 94235 , Cachan , France;3. MRC Laboratory of Molecular Biology , Hills Road, Cambridge , CB2 0QH , UK;4. Fondazione Istituto Pasteur-Fondazione Cenci Bolognetti, c/o Dipartimento di Genetica e Biologia Molecolare Università ‘La Sapienza’ , 00185 , Rome , Italy;5. Istituto Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche Università ‘La Sapienza’ , 00185 , Rome , Italy |
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| Abstract: | Abstract The contribution of histone-DNA interactions to nucleosome positioning in vivo is currently a matter of debate. We argue here that certain nucleosome positions, often in promoter regions, in yeast may be, at least in part, specified by the DNA sequence. In contrast other positions may be poorly specified. Positioning thus has both statistical and DNA-determined components. We further argue that the relative affinity of the octamer for different DNA sequences can vary and therefore the interaction of histones with the DNA is a ‘tunable’ property. |
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