Effect of Configuration of the Inhibitors on the Mode of Binding to the Enzyme,Thermolysin |
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Authors: | Indira Ghosh V SR Rao |
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Institution: | Molecular Biophysics Unit , Indian Institute of Science , Bangalore , India |
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Abstract: | Abstract Preferred conformations of the competitive inhibitors glycyl-L-phenylalanine and glycyl-D-phenylalanine and their mode of binding to thermolysin have been studied. The difference in configuration is shown to affect significantly the mode of binding to thermolysin. Gly-D-Phe prefers to enter the active site in the global minimum conformation whereas Gly-L-Phe may enter in a higher energy conformation. Moreover, D-enantiomer is shown to have a better fit than the L-counterpart in the active site. |
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