A General Procedure for Assigning the 31P Spectra of Drug-Oligonucleotide Complexes |
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| Authors: | Muriel Delepierre Bernard P Roques Béatrice Langlois D'Estaintot Jean Igolen |
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| Institution: | 1. Départment of Chimie Organique , U 266 INSERM, UA 498 CNRS UER des Sciences Pharmaceutiques et Biologiques , 4 avenue de l'Observatoire, 75006 , Paris , France;2. Unité de Chimie Organique Institut Pasteur , 25 rue du Docteur Roux, 75015 , Paris , France |
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| Abstract: | Abstract Taking advantage of the slow exchange at the NMR time scale occurring in drug oligonucleotides complexes the 3P signals in the bound forms are assigned by using 31PNMR two dimensional chemical exchange. This technique was applied to complexes between Actinomycin D and dCpGpCpG] or dm5CpGpm 5CpG], As compared to the labelled 17O,18O this method proved to be a powerful and unique way to assign 31P in broad spectrum or with long oligonucleotides. |
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