Kinetics of human lymphocyte division and chromosomal radiosensitivity |
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Authors: | N O Bianchi M S Bianchi M Larramendy |
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Affiliation: | Instituto Multidisciplinario de Biología Celular (IMBICE), Calle 526 entre 10 y 11, C.C. 403, 1900 La Plata Argentina |
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Abstract: | Human blood from normal donors was irradiated with 200 R during the Go phase, and the X-ray sensitivity of early and late dividing lymphocytes in culture was expressed as percentage of induced dicentrics. Cells in first or subsequent divisions were individualized by BrdU-Giemsa techniques. Lymphocytes in the first division at 40, 44 and 72 h after the start of culture had a lower sensitivity to radiation than lymphocytes making their first division at 48, 52 and 56 h. It was observed that: (a) the combination of radiation followed by BrdU did not increase the clastogenic action of X-rays, (b) X-rays in the dose and duration used in our cultures did not increase the frequency of SCEs, and (c) minor changes in culture conditions probably influenced the frequency of SCEs. |
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