Inhibition of Quorum Sensing in Serratia marcescens H30 by Molecular Regulation |
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| Authors: | H. Zhu Y. L. Shen D. Z. Wei J. W. Zhu |
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| Affiliation: | (1) State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, PRC;(2) Center of Bioengineering and Biotechnology, China University of Petroleum, Qingdao, 266555, PRC;(3) Chemical Engineering Research Center, East China University of Science and Technology, Shanghai, 200237, PRC |
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| Abstract: | Quorum sensing in Serratia marcescens, which uses two types of signaling molecules–N-acyl homoserine lactones and furanosyl borate diester–play important regulatory roles in the synthesis of 2,3-butanediol and prodigiosin. In the hope of understanding the effect of quorum sensing on physiologic metabolism, we established two molecular strategies, one to express acyl-homoserine lactone hydrolase to inactivate AI-1 signaling molecule using an expression vector with lactose as the inducer and the other to mutate luxS gene with a suicide plasmid pUTKm2 to inhibit the synthesis of AI-2 signaling molecule. |
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