Multiple domains mediate heme control of the yeast activator HAP1 |
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| Authors: | Maryann L. Haldi and Leonard Guarente |
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| Affiliation: | (1) Department of Biology, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA;(2) Present address: Whitehead Institute for Biomedical Research, 02142 Cambridge, MA, USA |
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| Abstract: | The activity of the yeast activator HAP1in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain, and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme. |
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