The effect of carbon source on callus induction and regeneration ability in Pharbitis nil |
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Authors: | Trejgell Alina Jarkiewicz Magdalena Tretyn Andrzej |
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Institution: | (1) Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń, 87-100, Gagarina 9, Poland |
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Abstract: | Flower buds, cotyledons and hypocotyls of Pharbitis nil were used as plant material. Flower buds (1–2 mm long) were excised from 3-week-old plants, grown in soil. Cotyledons of
7-day-old sterile seedlings were cut into 25 mm2 squares cotyledons whereas hypocotyls were cut to 1 mm long fragments. Explants were transferred into Petri dishes containing
the Murashige and Skoog medium (MS), supplemented with either BA (11 μM·L−1) alone or BA (22 μM·L−1) and NAA (0.55 μM·L−1), and different sugars: sucrose, fructose, glucose, mannose or sorbitol (autoclaved or filter-sterilized). Addition of glucose
instead of sucrose to the medium stimulated the induction of callus on flower buds and cotyledonary explants, but inhibited
its growth on fragments of hypocotyls. The medium supplemented with fructose (especially filter-sterilized) stimulated the
development of flower elements. Organogenesis of shoots and roots on explants was also observed. Flower buds and hypocotyls
were able to regenerate both organs. Addition of fructose or glucose to the medium stimulated the organogenesis of shoots,
whereas root organogenesis was inhibited on all explants used. Sorbitol strongly inhibited both induction of callus and organogenesis
on all explants used. |
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Keywords: | Pharbitis nil carbohydrate callus regeneration |
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