Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7 |
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Authors: | Han?Li Qunli?Zeng Yu?Weng Deqiang?Lu Huai?Jiang Email author" target="_blank">Zhengping?XuEmail author |
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Institution: | 1.Bioelectromagnetics Laboratory,Zhejiang University,Hangzhou,China;2.Research Center for Environmental Genomics,Zhejiang University School of Medicine,Hangzhou,China |
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Abstract: | Extremely Low Frequency Magnetic Fields (ELF MF) has been considered as a “possible human carcinogen” by International Agency
for Research on Cancer (IARC) while credible mechanisms of its carcinogenicity remain unknown. In this study, a proteomics
approach was employed to investigate the changes of protein expression profile induced by ELF MF in human breast cancer cell
line MCF7, in order to determine ELF MF-responsive proteins. MCF7 cells were exposed to 50 Hz, 0.4 mT ELF MF for 24 h and
the changes of protein profile were examined using two dimensional electrophoresis. Up to 6 spots have been statistically
significantly altered (their expression levels were changed at least 5 fold up or down) compared with sham-exposed group.
19 ones were only detected in exposure group while 19 ones were missing. Three proteins were identified by LC-IT Tandem MS
as RNA binding protein regulatory subunit Proteasome subunit beta type 7 precursor and Translationally Controlled Tumor Protein.
Our finding showed that 50 Hz, 0.4 mT ELF MF alternates the protein profile of MCF7 cell and may affect many physiological
functions of normal cell and 2-DE coupled with MS is a promising approach to elucidating cellular effects of electromagnetic
fields. |
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Keywords: | ELF MF MCF7 2-DE MS protein expression profile |
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