Ordered YAC Clone Contigs Assigned to Rice Chromosomes 3 and 11 |
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| Authors: | Tanoue Hiroshi; Shimokawa Takanori; Wu Jianzhong; Sue Norio; Umehara Yosuke; Ashikawa Ikuo; Kurata Nori; Sasaki Takuji |
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| Institution: | Rice Genome Research Program, National Institute of Agrobiological Resources 1-2 Kannondai, 2-chome, Tsukuba, Ibaraki 305, Japan/Institute of Society for Techno-Innovation of Agriculture, Forestry and Fisheries 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305, Japan |
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| Abstract: | Yeast artificial chromosome (YAC) clones were arranged on thepositions of restriction fragment length polymorphism (RFLP)and sequence-tagged site (STS) markers already mapped on thehigh-resolution genetic maps of rice chromosomes 3 and 11. Froma total of 416 and 242 YAC clones selected by colony/Southernhybridization and polymerase chain reaction (PCR) analysis,238 and 135 YAC clones were located on chromosomes 3 and 11,respectively. For chromosomes 3 and 11, 24 YAC contigs and islandswith total coverage of about 46% and 12 contigs and islandswith coverage of about 40%, respectively, were assigned. Althoughmany DNA fragments of multiple copy marker sequences could notbe mapped to their original locations on the genetic map bySouthern hybridization because of a lack of RFLP, the physicalmapping of YAC clones could often assign specific locationsof such multiple copy sequences on the genome. The informationprovided here on contig formation and similar sequence distributionrevealed by ordering YAC clones will help to unravel the genomeorganization of rice as well as being useful in isolation ofgenes by map-based cloning. |
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| Keywords: | physical map rice YAC chromosome contig |
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