The properties of the mitochondrial megachannel in mitoplasts from human colon carcinoma cells are not influenced by Bax |
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| Authors: | Campello Silvia De Marchi Umberto Szabò Ildikò Tombola Francesco Martinou Jean-Claude Zoratti Mario |
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| Affiliation: | CNR Institute of Neuroscience, Biomembranes Section, Department of Biomedical Sciences, University of Padova, Viale Giuseppe Colombo 3, 35121 Padova, Italy. |
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| Abstract: | This paper explores the relationship between Bax and the mitochondrial permeability transition pore (PTP). Isolated human colon tumor (HCT116) Bax- mitochondria exposed to recombinant Bax exhibited a slow, cyclosporin A-sensitive swelling, but only at [Bax]>200 nM. The amount of Bax incorporated was much higher than that found in organelles isolated from HCT116 Bax+ staurosporine- or etoposide-treated apoptotic cells, casting doubts on the significance of the putative PT induction for apoptosis. Bax did not influence the electrophysiological properties of an approximately 1 nS channel ascribed to the Ca2+-dependent mitochondrial permeability transition pore. These observations indicate that the PTP is independent of Bax. |
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| Keywords: | CSP, cyclosporin A CypD, cyclophilin D HCT, human colon tumor MMC, mitochondrial megachannel RLM, rat liver mitochondria PTP, permeability transition pore PS, phosphatidylserine pS, picoSiemen STS, staurosporine VDAC, voltage-dependent anion channel (porin) |
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