肠道需氧革兰氏阴性杆菌与门静脉血内毒素水平关系的初步探讨

本实验以普通 Webster 大鼠为动物模型,探讨肠道需氧革兰氏阴性杆菌(Gram ne-gative,G~-杆菌)与门静脉血内毒素的关系。以抗生素联合灌胃使大鼠肠道脱污染,降低肠道定植抗力,再以4种需氧 G~-杆菌混合液灌胃,行肠道再污染,然后再分别测定正常鼠(组),脱污染鼠(组)及再污染鼠(组)不同肠段和粪便需氧 G~-杆菌及相应鼠门静脉血内毒素水平。结果表明,肠道需氧 G~-杆菌的变化与相应门静脉血内毒素水平的变化基本一致.由此可见,上消化道需氧 G~-杆菌过生长,可能会成为门静脉血内毒素水平增加的原因之一。

Research on the Relationship between the Aerobic Gram-negative Bacteria in Intestine and Endotoxin in the Venous Blood

The relationship between the aerobic Gram-negative bacteria in intestine and the endotoxin in the blood stream of portal vein in Webster rats were studies.The intestinal tract of experimental rats were decontamina- ted by antibiotics and recontaminated with 4 different aerobic Gram-negative bacteria.The aerobic Gram-negative bacteria of different segments of the gut and faecal material were cultured and the estimation of endotoxin in the portal venous blood were made at the same time.The results indicated that the changes of aerobic Gram-negative bacteria always synchronize with the changes of endo- toxin level in the blood of the portal vein. Thus overgrowth of aerobic Gram-negative bacteria in the upper intestine may be one of the reasons in answering why there was an increasing in the endotoxin level in portal venous blood.