The fluorometric assay of rat plasma corticosterone: evaluation of nonspecific fluorescence |
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Authors: | John C. Butte Ryoko Kakihana Ernest P. Noble |
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Affiliation: | Department of Biology, San Francisco State University, San Francisco, California 94132 and National Institute on Alcohol Abuse and Alcoholism, 5600 Fishers Lane, Rockville, Maryland 20852 U.S.A. |
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Abstract: | An analysis of 168 plasma samples from intact rats, one to 35 days of age, was performed using both brief and specific fluorometric procedures. The amount of fluorescence produced by the brief procedure which could be attributed to corticosterone ranged from a maximum of 72% to a minimum of 16% of the total fluorescence value. Corticosterone represented 50% or more of the brief assay value in only five out of 18 groups of animals assayed. Following statistical analysis of the nonspecific fluorescence, a significant variation was found due to the age of the animal. A highly significant increase in nonspecific fluorescence was found in 21-day old animals following histamine injection. It was concluded that the brief fluorometric assays for corticosterone were of little value if specificity was desired. |
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