The in vivo metabolites of [14C] progesterone in bovine muscle and adipose tissue |
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Authors: | M.T. Lin V.L. Estergreen G.E. Moss J.D. Willett W. Shimoda |
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Affiliation: | Department of Animal Sciences Washington State University, Pullman, WA 99164 U.S.A.;Department of Chemistry University of Idaho, Moscow, ID 83843 USSR;Food and Drug Administration Agricultural Research Center, Beltsville, MD 20705 U.S.A. |
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Abstract: | Muscle and adipose tissue were obtained from steers and dairy cows following subcutaneous administration of [14C] progesterone. Following extraction, purification and separation by column, thin layer and gas-liquid chromatography, various radioactive residues from these tissues were identified by their Chromatographic mobility, crystallization to constant specific activity and mass spectra. Progesterone constituted 54% of free radioactivity extracted from muscle and 69 and 73% of radioactivity in the free and conjugated portions of extracts, respectively, from fat. Metabolites identified were: 5α-pregnane-3,20-dione, 9%, 0%, 0%, 20β-hydroxy-4-pregnen-3-one, 8%, 11%, 3%; 3α-hydroxy-5β-pregnan-20-one, 13%, 2%, 2%; 3α-hydroxy-5α-pregnan-20-one, 3%, 3%, 6%; 20α-hydroxy-5α-pregnan-3-one, 0%, 2%, 3%; of radioactivity in muscle (free) and fat (free and conjugated fractions), respectively. Tentatively identified in fat extracts by chromatographic mobility were: 20α-hydroxy-4-pregnen-3-one, 1%, 1% and 3β-hydroxy-5β-pregnan-20-one, 0%, 2% of radioactivity in free and conjugated fractions, respectively. The average concentration of steroid in these animals due solely to treatment, calculated from the specific activity of the [14C] progesterone administered, was 3.4 and 18.1 ng/g in muscle and subcutaneous fat, respectively. |
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Keywords: | Reprint request: V. L. Estergreen Department of Animal Sciences Washington State University Pullman WA 99164. |
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