促性腺激素释放激素类似物对长臀鮠脑垂体促性腺激素分泌的影响

采用离体灌流孵育技术和促性腺激素的放射免疫测定方法，对长臀鮠(Cranoglanis bouderius)脑垂体碎片促性腺激素的分泌进行了研究。结果表明：持续的促性腺激素释放激素类似物(GnRH-A)能显著刺激退化期的长臀鮠离体脑垂体碎片促性腺激素(GTH)的分泌，并且长臀鮠脑垂体碎片对持续的GnRH-A刺激未表现出脱敏性，该结果与胡子鲇和鲇鱼相似，而与金鱼和鲤科鱼类不同；重复脉冲GnRH-A刺激对长臀鮠脑垂体碎片GTH分泌具有促进作用，而且存在剂量依存关系，与鲇鱼和鲤科鱼类相类似。上述结果表明在长臀鮠的人工繁殖中可以用持续高浓度GnRH-A刺激对长臀鮠进行催熟和催产。

Effect of Gonadotropin Hormone-Releasing Hormone Analogs on the Gonadotropin Secretion by the Pituitary of Helmet Catfish (Cranoglanis bouderius) in vitro

The gonadotropin (GTH) secretion from pituitary fragments of helmet catfish (Cranoglanis bouderius) was studied by a perfusion incubation system and GTH radiommunoassay (RIA).The results showed that chronic administration of gonadotropin hormone-releasing hormone analogs (GnRH-A) was able to stimulate GTH release from pituitary fragments in vitro,which was consistent to African catfish (Clarias gariepinus) and catfish (Silurus asotus).The insensitivity to chronic administration of GnRH-A was not be observed in the helmet catfish,which was consistent to catfish and African catfish,but different from that in goldfish (Carassius auratus) and Cyprinus carpio haematopters.Repetitive pulse administration of GnRH-A was also able to stimulate GTH release from the pituitary fragments in dose-dependent manner in helmet catfish.These results showed that chronic administration of GnRH-A could have an effect on artificial propagation of helmet catfish.