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The sequence of a porcine cDNA encoding α-lactalbumin
Authors:Neil A Das Gupta  Leeson J Alexander  Craig W Beattie
Abstract:A cDNA clone encoding porcine α-lactalbumin (αLA) was isolated and sequenced. The longest clone was 688 nucleotides (nt) long and encoded a preprotein of 141 amino acids (aa) including a leader peptide of 19 aa. The porcine cDNA exhibited a nt similarity of between 72.2%–83.5% to other αLA cDNAs and an aa similarity of between 50.8%–85.2% with other αLA aa sequences. The derived aa sequence varied at three positions from a previously reported sequence for porcine αLA obtained by direct aa sequencing.
Keywords:Recombinant DNA  phage λ  libraries  whey  milk proteins  lactation  sus scrofa  swine  artiodactyl
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