Inhibition of nitric oxide and soluble guanylyl cyclase signaling affects olfactory neuron activity in the moth, <Emphasis Type="Italic">Manduca sexta</Emphasis> |
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Authors: | Caroline H Wilson Thomas A Christensen Alan J Nighorn |
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Institution: | (1) Arizona Research Laboratories, Division of Neurobiology, University of Arizona, Tucson, AZ 85721, USA;(2) Pacific Biomedical Research Center, Békésy Laboratory of Neurobiology, University of Hawai’i, Honolulu, HI 96822, USA |
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Abstract: | Nitric oxide is emerging as an important modulator of many physiological processes including olfaction, yet the function of
this gas in the processing of olfactory information remains poorly understood. In the antennal lobe of the moth, Manduca sexta, nitric oxide is produced in response to odor stimulation, and many interneurons express soluble guanylyl cyclase, a well-characterized
nitric oxide target. We used intracellular recording and staining coupled with pharmacological manipulation of nitric oxide
and soluble guanylyl cyclase to test the hypothesis that nitric oxide modulates odor responsiveness in olfactory interneurons
through soluble guanylyl cyclase-dependent pathways. Nitric oxide synthase inhibition resulted in pronounced effects on the
resting level of firing and the responses to odor stimulation in most interneurons. Effects ranged from bursting to strong
attenuation of activity and were often accompanied by membrane depolarization coupled with a change in input resistance. Blocking
nitric oxide activation of soluble guanylyl cyclase signaling mimicked the effects of nitric oxide synthase inhibitors in
a subset of olfactory neurons, while other cells were differentially affected by this treatment. Together, these results suggest
that nitric oxide is required for proper olfactory function, and likely acts through soluble guanylyl cyclase-dependent and
-independent mechanisms in different subsets of neurons. |
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Keywords: | Olfaction Nitric oxide Soluble guanylyl cyclase Odor coding Insect |
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