| 大鼠精核蛋白抗血清的制备 |
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| 引用本文： | 戴文平,左晓春,陈晖,费仁仁.大鼠精核蛋白抗血清的制备[J].中国生物化学与分子生物学报,1994,10(5):561-564. |
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| 作者姓名： | 戴文平 左晓春 陈晖 费仁仁 |
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| 作者单位： | 中国医学科学院基础医学研究所，中国医学科学院药物研究所 |
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| 摘 要： | 用大鼠精核蛋白（Ｒａｔ Ｐｒｏｔａｍｉｎｅ，ＲＰ）－核糖核酸（ＲＮＡ）复合物（ＲＰ－ＲＮＡ Ｃｏｍｐｌｅｘｅｓ）免疫大鼠，得到了特异的抗ＲＰ抗血清，并用Ｉｍｍｕｎｏｄｏｔｔｉｎｇ和Ｉｍｍｕｎｏｂｌｏｔｔｉｎｇ方法验证了其特异性。该抗血清和ＲＰ有特异的反应，并和哺乳动物小鼠和羊的精核蛋白有一定程度交叉反应，而与体细胞类型核蛋白（Ｈ１，Ｈ２ａ，Ｈ２ｂ，Ｈ３，Ｈ４）无交叉反应。并对制备该抗血清的意义予以讨论。
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| 关 键 词： | 大鼠精核蛋白 精子细胞 精子发生 |
| 收稿时间： | 1994-10-20 |
Preparation of Antisera Against Rat Protamine |
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| Dai,Wen-ping,Zuo,Xiao-chun,Chen,Hui,Fei,Ren-ren.Preparation of Antisera Against Rat Protamine[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(5):561-564. |
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| Authors: | Dai Wen-ping Zuo Xiao-chun Chen Hui Fei Ren-ren |
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| Institution: | (Chinese Academy of Medical Sciences,Beijing 100005 |
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| Abstract: | Specific polyclonal antisera against rat protamine (RP) using RP-RNA complexes as an antigen have been obtained. The specificity of antibodies was determined in immunedotting and immunoblotting assays. The antisera reacted specifically with RP, but not with somatic nuclear proteins, the antisera may cross-react with mouse and ram protamines. The significance of antisera against protamine was discussed. |
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| Keywords: | Rat protamine Spermatids Spermiogenesis |
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