Intracellular nuclease activities of buffalo rumen bacterial population |
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Authors: | P R Sinha S M Dutta |
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Institution: | (1) Department of Dairy Chemistry, National Dairy Research Institute, 132 001 Karnal, India |
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Abstract: | Nuclease activities of the predominantly bacterial population obtained from buffalo rumen were investigated. Optimum temperature
for hydrolysis of both DNA and RNA was 50°C whereas DNAase activity was observed to be stable up to 50°C, a decrease in RNAase
activity was observed even after 40°C. Two pH optima, one at 5.5 and the other at 7.5, were recorded for hydrolysis of DNA.
RNAase activity was maximum between pH 6.0 to 7.0. Whereas DNAase activity was stable near its optimum pH, RNAase activity
was stable between pH 7.0 to 8.5. Mn2+ ions stimulated DNAase activity. It was strongly inhibited by Hg2+, Zn2+, Pb2+ and Ag+. RNAase activity was stimulated by Mg2+ ions and was strongly inhibited by Hg2+, Cu2+, Zn2+ and Ag+. Cysteine hydrochloride and 2-mercaptoethanol stimulated DNAase activity. The activity was strongly inhibited by N-ethylmaleimide,
4-chloromercuribenzoate, 8-quinolinol, iodoacetic acid and 1,10-phenanthroline. RNAase activity was stimulated by cysteine
hydrochloride, reduced glutathione and 2-mercaptoethanol and was strongly inhibited by 4-chloromercuribenzoate, 8-quinolinol
and 2,2′-bipyridyl.
Part of PhD Thesis submitted by the first author to Kurukshetra University. |
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