Purification and Characterization of Sucrose Synthetase from the Shoot of Bamboo Leleba oldhami |
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| Authors: | Su J C |
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| Institution: | Department of Agricultural Chemistry, National Taiwan University, and Institute of Biological Chemistry, Academia Sinica. |
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| Abstract: | A 108-fold purification of the sucrose synthetase from the extract of the shoot of bamboo Lelaba oldhami was achieved by ammonium sulfate fractionation, calcium phosphate gel adsorption, and chromatographic separations on Sephadex G-100 and diethylaminoethyl-cellulose columns. Some properties of this enzyme, namely thermal and pH stabilities, stabilization by aqueous glycerol, pH optimum, substrate specificities, effects of metallic ions, effects of sulfhydryl reagents, molecular weight, sedimentation constants, isoelectric point, and substrate saturation kinetics had been investigated. |
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