Shoot Regeneration from Fragmented Flower Buds of Carnation (Dianthus caryophyllus) |
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Authors: | MILLER ROBYN M; KAUL VIJAY; HUTCHINSON JAMES F; MAHESWARAN GOWRI; RICHARDS DENNIS |
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Institution: | Institute of Plant Sciences, Department of Agriculture P.O. Box 174, Ferntree Gully, Victoria 3156, Australia |
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Abstract: | Adventitious shoots were regenerated from fragmented flowerbuds, individual petals and receptacles in a number of differentcarnation cultivars. The major site of shoot formation was thesubepidermal cells at the proximal end of the petals. The yieldof shoots from a single flower bud was high, ranging between70 and 275, for the 11 cultivars tested. The regeneration mediumcontained Murashige and Skoog basal medium supplemented with48 µm -naphthaleneacetic acid and 48 µmbenzyladenine. The preferred regeneration protocol appears highlysuited to the development of gene transfer systems. Adventitious shoots, Dianthus caryophyllus L., tissue culture, explant, auxin, cytokinin, cut flowers, floriculture, organogenesis |
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