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| 壳聚糖对嘧啶核苷类新型抗真菌抗生素发酵液絮凝除菌作用的研究 | |
| 引用本文： | 孟强,梁雪琴,陈华,贺娟,吴绵斌,陈正杰.壳聚糖对嘧啶核苷类新型抗真菌抗生素发酵液絮凝除菌作用的研究[J].工业微生物,2010,40(5):45-48. |
| 作者姓名： | 孟强 梁雪琴 陈华 贺娟 吴绵斌 陈正杰 |
| 作者单位： | 1. 浙江海正药业股份有限公司,台州,318000;浙江省抗真菌药物研究重点实验室,台州,318000 2. 浙江大学化学工程与生物工程系生物工程研究所,杭州,310027 3. 浙江大学化学工程与生物工程系生物工程研究所,杭州,310027;浙江海正药业股份有限公司,台州,318000;浙江省抗真菌药物研究重点实验室,台州,318000 |
| 基金项目： | 浙江省科技厅计划项目，台州市-浙江大学科技合作专项资金项目 |
| 摘 要： | 利用天然高分子聚合物壳聚糖为絮凝剂去除抗生素发酵液中的菌体,结果表明:发酵液pH值、絮凝剂用量是影响絮凝效果的主要因素。最佳絮凝条件为pH7.0,絮凝剂用量0.4g/L,温度30℃,此时菌体絮凝率(FR%)可达70%以上。经絮凝预处理后,滤速为处理前的2.5倍,发酵液的效价由700 U/mL提高到860 U/mL。 |
| 关 键 词： | 壳聚糖 絮凝 嘧啶核苷 抗真菌抗生素 |
Cell debris flocculation in culture broth containing a novel nucleoside antifungal antibiotic by chitosan |
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| Meng Qiang,LIANG Xue-qin,CHEN Hua,HE Juan,WU Mian-bin,CHen Zheng-jie.Cell debris flocculation in culture broth containing a novel nucleoside antifungal antibiotic by chitosan[J].Industrial Microbiology,2010,40(5):45-48. | |
| Authors: | Meng Qiang LIANG Xue-qin CHEN Hua HE Juan WU Mian-bin CHen Zheng-jie |
| Institution: | 1. Institute of Bioengineering, Zhejiang University, Hangzhou 310027; 2. Zhejiang Hisun Pharmaceutical Co. , Ltd. ,Taizhou 318000; 3. Zhejiang Key Laboratory of Antifungal Drugs, Taizhou 318000) |
| Abstract: | To remove cell debris in culture broth of a nucleoside antifungal antibiotic, chitosan was choosed as flocculant in this study. The optimum flocculation conditions obtained were as follows： pH was 7.0, the added flocculant （ehitosan） was 0.4 g/L and temperature was 30 ℃. Under the optimized conditions, the flocculating rate （FR） of cell debris was above 70 %. After treated with chitosan, filtration rate was 2.5 fold than that of the untreated control and the antibiotic potency was improved from 700 U/mL to 860 U/mL. |
| Keywords: | chitosan flocculation nucleoside antifungal antibiotic |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |