几种激素在鳜胃肠道内分泌细胞中存在的免疫细胞化学证据

用链酶亲和素－生物素过氧化物酶（Strept Avidin-Biotin-Complex,SABC）免疫细胞化学方法，使用4种兔抗消化 道激血清对鳜胃肠道中的内分泌细胞进行鉴别和定位。结果在鳜鱼胃的贲门、幽门及肠道中均发现不同程度地存在着生长抑素和五羟色胺免疫活性内分泌细胞。在鳜的贲门上皮和贲门腺之间，幽门上皮和幽门腺之间均分布有生长抑素免疫活性阳性细胞，在贲门腺和幽门腺处的分布较密。这些含五羟色胺的肽能神经元具有典型的APUD（Amine Precursor Uptake and Decarboxylation）细胞特征，提示鱼类的胃肠道同哺乳动物的胃肠道一样富含肽能神经元；为神经－内分泌系统的研究提供有力的形态学依据，另外两种抗血清－高血糖素和胃泌素的免疫细胞化学染色在鳜的胃肠道的各部位均未发现阳性反应。

THE IMMUNOCYTOCHEMICAL EVIDENCE FOR TWO HORMONES IN THE GASTROINTESTINAL ENDOCRINE CELLS OF MANDARIN FISH

By using immunocytochemical method of Strept Avidin-Biotin-Complex(SA BC), 4 kinds of antisera raised against rabbit hormones were used to identify and locate the endocrine cells in the gastrointestinal tract of mandarin fish(Siniperca chuatsi). Results showed that glucagon(Glu) -and gastrin(Gas) - immunoreactive endocrine cells were not found in the gastrointestinal tract of mandarin fish; somatostatin(Som) - , and serotonin(5- HT) -immunoreactive endocrine cells were found in the cardia, pylorus and gut of mandarin fish in varying degrees. There were distributions of Som-immunoreactive endocrine cells between epithelium cardia and cardiac gland, and between epithelium pylorus and pyloric gland; their distribution in the cardiac gland and pyloric gland was denser than in the epithelium cardia and pylorus. There were more 5 - HT-immunoreactive endocrine cells in the fundus vertriculi and intestinal epithelial tissue. Also there were more 5 - HT-immunoreactive endocrine cells in the neurones of nerve plexus in muscle layer. These peptidergic neurones contained serotonin have the typical characteristic of APUD(Amine Precursor Uptake and Decarboxylation) cells. It is a prompt that as in the mamalian also there are many peptidergic neurones in the gastrointestinal tract of fish. It provided morphological basis for the research of neuro-endocrine system.