Genetic and virulence characterization of <Emphasis Type="Italic">Streptococcus suis</Emphasis> type 2 isolates from swine in the provinces of Zhejiang and Henan,China |
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Authors: | Yulong Tang Huancan Zhao Wei Wu Di Wu Xiaoliang Li Weihuan Fang |
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Institution: | (1) Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310029, China; |
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Abstract: | This study was aimed to examine the genetic characteristics of 44 Streptococcus suis type 2 (SS2) isolates and the virulence attributes of 23 representative isolates. Multilocus sequence typing revealed five
sequence types (ST1, ST7, ST28, ST86, and ST162) with 19 isolates assigned to ST7 (43.2%), 14 to ST1 (31.8%), and 9 to ST28
(20.5%). PCR typing of the pilus gene clusters classified the isolates into three types: A (72.7%), B (22.7%), and N (4.5%).
All isolates of pilus types A and N were assigned to the ST1 complex containing ST1, ST7, and ST86, while those of type B
belonged to the ST27 complex comprising ST28 and ST162. Only two strains had the putative pathogenicity island 89-kb cluster
(89K) and were of type N. The type B strains had a significantly lower adhesion, were more readily killed by macrophage, and
had lower virulence to mice than those of types A and N. We conclude that SS2 strains of both ST1 and ST27 complexes, parallel
to pilus types A and B, were prevalent in the pig populations in Zhejiang Province, and ST7 and ST1 strains were the predominant
genotypes in the diseased pigs with pneumonia. |
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