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Development and characterization of 20 polymorphic microsatellite markers for Livistona chinensis R. Br. var. subglobosa Becc., a fan palm in the Ryukyu Archipelago,Japan
Authors:Toshiaki Kondo  Rie Kajita  Toshihiro Yamada
Institution:1. Graduate School for International Development and Cooperation, Hiroshima University, Higashi‐Hiroshima, 1‐5‐1 Kagamiyama, Japan;2. Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi‐Hiroshima, 1‐7‐1 Kagamiyama, Japan;3. Graduate School of Integrated Arts and Sciences, Hiroshima University, Higashi‐Hiroshima, 1‐7‐1 Kagamiyama, Japan
Abstract:Livistona chinensis R. Br. var. subglobosa Becc. is a subtropical fan palm distributed mainly in the islands of the Ryukyu Archipelago, Japan. Some small populations, separated by a few 100 km from the main species range, are also established on islands around northern Kyushu and Cape Ashizuri, Shikoku. Most of those are designated as national or prefectural natural treasures. We developed 20 microsatellite markers for this species and characterized them for polymorphisms within six populations. The number of alleles per locus ranged from two to nine, with an average of 4.2; the expected heterozygosity ranged from 0.16 to 0.61, with an average of 0.46. These markers will be useful for investigating disjunct range formation within L. chinensis var. subglobosa and the origins of the remote populations, providing crucial information for conservation of the populations.
Keywords:   Arecaceae  microsatellite  molecular marker  phylogeography
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