Development and characterization of EST‐SSR markers in an East Asian temperate plant genus Diabelia (Caprifoliaceae) |
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| Authors: | Kun‐Kun Zhao Hua‐Feng Wang Shota Sakaguchi Sven Landrein Yuji Isagi Masayuki Maki Zhi‐Xin Zhu |
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| Institution: | 1. Ministry of Education, College of Horticulture and Landscape Architecture, Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resource, Hainan University, 58 Renmindadao Road, Haikou 570228, China;2. Graduate School of Human and Environmental Studies, Yoshida‐nihonmatsu‐cho, Sakyo‐ku, Kyoto 606‐8501, Japan;3. Royal Botanic Gardens, Surrey TW9 3AB, UK;4. Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake‐cho, Sakyo‐ku, Kyoto 606‐8502, Japan;5. Botanical Gardens, Tohoku University, Kawauchi 12–2, Aoba, Sendai 980‐0862, Japan |
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| Abstract: | A set of expressed sequence tag (EST) simple sequence repeat (SSR) markers were developed and characterized using next‐generation sequencing technology for the genus Diabelia (Caprifoliaceae). De novo assembly of RNA‐seq reads resulted in 58 669 contigs with the N50 length of 1211 bp. A total of 2746 contigs were identified to harbor SSR motifs, of which 48 primer pairs were designed and 11 were shown to be polymorphic across three morphospecies of Diabelia. When evaluated with 30 individuals, the number of alleles per locus ranged from 2 to 11 and the expected heterozygosity varied from 0.399 to 0.873, respectively. Distance‐based clustering indicated that the EST‐SSR markers can provide sufficient power to distinguish the three species (or populations). These markers will be useful for evaluating the range‐wide genetic diversity of each species and examining genetic divergence and gene flow between the three species. |
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| Keywords: |
Caprifoliaceae
Diabelia
EST‐SSR marker next‐generation sequencing technology |
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