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| 血管紧张素Ⅱ在小鼠卵泡闭锁中的作用 | |
| 作者姓名： | Cheng Y Jiao LH Liu RH Wang QB Wang H Xia GL |
| 作者单位： | 1. 中国科学院动物研究所计划生育生殖生物学国家重点实验室,北京,100080;中国农业大学生物学院,北京,100094 2. 中国科学院动物研究所计划生育生殖生物学国家重点实验室,北京,100080 3. 中国农业大学生物学院,北京,100094 |
| 基金项目： | ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 39670 761) . |
| 摘 要： | 应用幼年小鼠经孕马血清促性腺激素（pregnant mares serum gonadotropin,PMSG)处理的动物模型,研究了卵泡从发育到闭锁动态变化过程中血管紧张素Ⅱ（AngⅡ）的作用。结果表明：（1）24日龄小鼠给予PMSG（10IU/只）后6d时,卵巢中出现大量闭锁卵泡,颗粒细胞DNA琼脂糖电泳显示了梯形条带;（2）随卵泡闭锁发生,卵巢AngⅡ含量增加;（3）AngⅡ显著拮抗FSH刺激颗粒细胞雌二醇生成的作用。我们认为,AngⅡ参与了对小鼠卵泡闭锁的调节。 |
| 关 键 词： | 血管紧张素Ⅱ 卵泡闭锁 颗粒细胞 雌二醇 |
| 修稿时间： | 2001年5月30日 |
Effect of angiotensin II on follicular atresia in mouse |
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| Cheng Y,Jiao LH,Liu RH,Wang QB,Wang H,Xia GL.Effect of angiotensin II on follicular atresia in mouse[J].Acta Physiologica Sinica,2002,54(1):75-78. | |
| Authors: | Cheng Yong Jiao Li-Hong Liu Rui-Hua Wang Qing-Bin Wang Hong Xia Guo-Liang |
| Institution: | State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, mwangh@panda.ioz.ac.cn |
| Abstract: | The effect of angiotensin II (Ang II) on the follicular development was studied by using an animal model of follicular atresia induced by pregnant mare s serum gonadotropin (PMSG). The results showed that: (1) a large number of atretic follicles were found in the ovary of 24-day-old mouse after 6-day treatment of PMSG. Deoxyribonucleic acid (DNA) extracted from granulosa cells clearly showed a ladder band under agarose gel electrophoresis analysis. (2) the contents of Ang II in the ovary extremely increased with the development of follicular atresia. (3) Ang II significantly antagonized the stimulating effect of the follicle-stimulating hormone (FSH) on estradiol (E(2)) generation of granulosa cells. It is suggested that Ang II may be involved in the regulation of follicular atresia in mouse. |
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