Accumulation of hydroxyapatite in the kidney of streptozotocin-induced diabetic rat fed a low-zinc diet |
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| Authors: | Takeshi Minami Yuko Okazaki Hisanao Komiya Yoshitaka Horiuchi Toru Inoue Yuya Yamada Hisako Fushimi |
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| Institution: | (1) Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, Higashi-Osaka, 577 Osaka;(2) Department of Electron Microscope, Institute of Life Science, Kinki University, Osaka, Japan;(3) Department of Internal Medicine, Sumitomo Hospital, Osaka, Japan |
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| Abstract: | Calcification occurred in the kidney of streptozotocin (STZ)-induced diabetic rats fed a low-zinc diet. The deposits were
stained by the von Kossa method and were detected intracellularly in the tubular cells, mainly in the cortico-medullar region.
The deposits were found to be a heterogenous substance on electron microscopy. There were various sizes of deposits, and the
surfactant was very much distorted. Many deposits grew up to bind small particles, and the core-like substance was observed
in the center of the deposit. The component of the deposit was analyzed by an X-ray microanalyzer, and was found to be calcium
and phosphorus. The ratio of calcium to phosphorus was 2.159, which coincided with the ratio of standard hydroxyapatite. From
these observations, the deposit is believed to be hydroxyapatite. It is thought that the core is formed at first, many particles
are then bound to the core, and deposits grow up. |
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| Keywords: | Nephrocalcinosis hydroxyapatite diabetic rat streptozotocin low-zinc diet X-ray microanalyzer |
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