The effect of gyrase inhibitors and cyclic AMP on induction and glucose repression of the 6-hydroxy-nicotine oxidases in Arthrobacter oxidans |
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Authors: | Roderich Brandsch Karl Decker |
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Institution: | (1) Biochemisches Institut der Albert-Ludwigs-Universität, Hermann-Herder-Str. 7, D-7800 Freiburg, Germany |
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Abstract: | The induction by d,l-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Archrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but led to an increase in the level of 6-hydroxy-D-nicotine oxidase activity. Furthermore, the specific repression by glucose of 6-hydroxy-D-nicotine oxidase synthesis was not abolished by the addition of cAMP but by that of novobiocin.Abbreviations 6-HDNO
6-hydroxy-D-nicotine oxidase
- 6-HLNO
6-hydroxy-L-nicotine oxidase
- cAMP
cyclic 3,5-adenosine monophosphate
- Enzymes
Adenylate cyclase
- ATP
pyrophosphate-lyase (cyclizing) (EC 4.6.1.1)
- cAMP-phosphodiesterase
3:5-cyclic-nucleotide 5-nucleotido-hydrolase (EC 3.1.4.17)
- DNA gyrase
DNA topoisomerase II (EC 5.99)
- DNA polymerase
deoxynucleosidetriphosphate: DNA desoxynucleotidyl-transferase (EC 2.7.7.7)
- 6-hydroxy-L-nicotine oxidase
6-hydroxy-L-nicotine: oxygen oxidoreductase (EC 1.5.3.5)
- 6-hydroxy-D-nicotine oxidase
6-hydroxy-D-nicotine: oxygen oxidoreductase (EC 1.5.3.6)
- -lactamase
penicillin amido--lactamhydrolase (EC 3.5.2.6)
- nicotine dehydrogenase
nicotine: (acceptor)6-oxidoreductase (hydroxylating) (EC 1.5.99.4) |
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Keywords: | Arthrobacter oxidans cAMP Catabolite repression DNA gyrase Nalidixic acid Novobiocin |
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