Cation dependent conformations of brain CA2+-dependent regulator protein detected by nuclear magnetic resonance. |
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Authors: | K Seamon |
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Affiliation: | Department of Psychiatry Washington University School of Medicine Saint Louis, Missouri 63110 USA |
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Abstract: | The 250MHz NMR spectrum of the brain Ca2+-dependent regulator protein was examined in the absence of cations and in the presence of Ca2+ or Mg2+. The Ca2+-saturated regulator protein and Mg2+-saturated regulator protein exhibited several spectral differences in the aromatic and aliphatic regions of their spectra. Certain spectral changes observed to occur upon addition of metal ions are qualitatively similar to those which have been observed in the spectrum of skeletal troponin-C. These results suggest that the large sequence homology between skeletal troponin-C and the regulator protein results in similar conformational changes due to the binding of Ca2+ or Mg2+. |
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Keywords: | EGTA ethylene glycol bis(βOaminoethyl ether)-N,N′-tetraacetic acid TSP trimethylsilylproprionic acid tml trimethyllysine |
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