DNA splicing of mitochondrial group I and II introns in Schizosaccharomyces pombe |
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Authors: | Ana Maria Merlos-Lange Feryal Kanbay Manfred Zimmer Klaus Wolf |
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Affiliation: | (1) Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Straße 1 a, D-8000 München 19, Federal Republic of Germany |
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Abstract: | Summary In this paper we report the precise excision of the group I intron aI2b from the cox1 gene and of the group II intron bI from the cob gene fo the Schizosaccharomyces pombe strain 50. We present evidence that DNA excision of both intron DNA sequences is under nuclear control. Attempts to remove the first cox1 intron (aI1) have failed so far, but a deletion of approximately 200 bp in the open intronic reading frame demonstrates that it is not essential for normal cellular functions.Abbreviations cox1, cox2, cox3 genes encoding subunits 1, 2 and 3 of cytochrome c oxidase - cob gene encoding apocytochrome b - rns and rnl genes encoding the small and large ribosomal RNA - atp6, atp8 and atp9 genes encoding subunits 6, 8, and 9 of the ATP synthase complex - urfa unassigned reading frame a - aI1, aI2a, aI2b, aI3 introns in the cox 1 gene of S. pombe - bI intron in the cob gene - del-aI2b and del-bI respiratory competent strains in which the respective introns have been deleted by DNA splicing |
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