The artificial fecundation in Japanese monkeys (Macaca fuscata) by induced ovulation and artificial insemination in nonmating season]

Induced ovulation trials by PMSG-hCG administration were conducted in eleven female Japanese monkeys showing neither bleeding nor ovulation in nonmating season. The ovulation was confirmed by the laparoscopic observation in nine of the 11 females. Artificial inseminations were performed in these 9 females by the injection of semen collected by the penile electrode approach. The semen was injected into the uterine cervix in 6 females or the uterine cavity in 3 females. A gestational sac was confirmed on the ultrasonic diagnostic apparatus 19 days after ovulation in one of the 3 females inseminated into the uterine cavity. The pregnancy, however, could not be maintained.