Cytochemical localization of glycolate oxidase in microbodies of Klebsormidium |
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Authors: | P. J. Gruber S. E. Frederick |
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Affiliation: | (1) Department of Biological Sciences, Mount Holyoke College, 01075 South Hadley, MA, USA |
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Abstract: | The filamentous green alga Klebsormidium flaccidum A.Br. was fixed with glutaraldehyde, incubated in a cytochemical medium designed to detect glycolate-oxidase activity, and prepared for electron microscopy. Heavy deposits of stain were observed in microbodies following incubation with either glycolate or L-lactate as substrate, but not after incubation with D-lactate or H2O. When Chlamydomanas reinhardi Dangeared cells were treated in the same way, their microbodies did not appear stained. The results establish that in Klebsormidium glycolate-oxidase occurs in microbodies (peroxisomes), as it does in angiosperms; also, they emphasize the dichotomy between those green algae which contain glycolate-oxidase and those, such as Chlamydomonas, which possess the mitochondrial enzyme glycolate dehydrogenase. |
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Keywords: | Cytochemistry Glycolate oxidase Klebsormidium Peroxisomes |
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