Cd2+、Al3+作用下蚕豆UDS与微核相关性分析及高等植物UDS技术初探

为探讨金属离子对高等植物非按期DNA合成(UnscheduledDNASythesis,简称UDS)和微核(MCN)的诱导作用、二者之间的关联性以及利用高等植物UDS技术检测环境诱变物的可行性,利用3HTdR前体掺入法研究了Cd²⁺、Al³⁺作用下蚕豆的UDS效应。结果表明,Cd²⁺、Al³⁺均能不同程度地诱导蚕豆UDS和MCN的发生;UDS量与微核率(MCNF)之间呈负相关(r<0),但相关不显着(|r|0.05),且二者间的相关程度在Cd²⁺和Al³⁺两种金属离子作用下没有显着差别(P>0.05);利用高等植物UDS技术检测环境诱变物质,在一定受检物剂量范围内是可靠的,但超过这个剂量范围,UDS技术无法检出.

Correlation analysis between UDS and MCN in Vicia faba treated with Cd2+ and Al3+ and UDS technique in higher plants

With the method of inserting 3H TdR into DNA, this paper studied the inductive effect of Cd 2 and Al 3 on unscheduled DNA synthesis (UDS) and micronucleus (MCN) in Vicia faba , the correlation between UDS and MCN, and the feasibility of using higher plant UDS test as the indicator to detect environmental mutagens. The results indicated that both Cd 2 and Al 3 could induce UDS and MCN in Vicia faba . There was a negative relationship between UDS and MCN, but it was not significant. The relationship between UDS and MCN was similar under the treatments of Cd 2 and Al 3 respectively. It was reliable using higher plant UDS test as the indicator to detect environmental mutagens within certain dosage range.