The staining of sciatic nerve glycoproteins on polyacrylamide gels with concanavalin A-peroxidase. |
| |
Authors: | J G Wood F O Sarinana |
| |
Institution: | 1. Department of Anatomy, The University of Tennessee Center for the Health Sciences, Memphis, Tennessee 38163 USA;2. Division of Neurosciences, City of Hope National Medical Center, Duarte, California 91010 USA |
| |
Abstract: | The plant lectin concanavalin A (Con A) possesses a remarkably specific capacity to bind primarily α-d-mannose or α-d-glucose sugar residues on macromolecules (cf. 1). The multivalent Con A will bind to carbohydrates on cell surfaces, and free binding sites on the attached Con A will bind to horseradish peroxidase which is a glycoprotein (2). Since peroxidase may be visualized by reaction with diaminobenzidine (3), it has been possible using this method to specifically “stain” carbohydrate residues on cell surface macromolecules (2, 4). The same principles for staining cell surfaces should apply to “staining” glycoproteins separated by polyacrylamide electrophoresis. In this paper, we examine the staining of glycoproteins in sciatic nerve by a Con A-peroxidase labeling technique. The method is more sensitive for mannose or glucose containing glycoproteins than the periodic acid-Schiff's (PAS) method commonly used. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|