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Stage-Specific Changes in Calcium-Regulated Protein Phosphorylation in Developing Tomato Fruits
Authors:Raghothama  K G; Veluthambi  K; Poovaiah  B W
Institution:Department of Horticulture and Landscape Architecture, Washington State University Pullman, WA 99164-6414, U.S.A.
Abstract:The role of calcium and calmodulin in the in vitro phosphorylationof soluble and membrane proteins was studied in relation togrowth and development of tomato fruits. Calcium at micromolarconcentrations promoted the phosphorylation of both solubleand membrane proteins. The calmodulin antagonists, chlorpromazineand trifluoperazine, inhibited the phosphorylation of severalproteins. Qualitative changes were observed in the pattern ofprotein phosphorylation at different developmental stages. Therewas a general decrease in protein phosphorylation towards ripening.These results indicated that calcium may be involved in theregulation of phosphorylation of different proteins at differentstages of fruit development. 1 Scientific Paper No. 7149, College of Agriculture and HomeEconomics, Washington State University, Pullman, Project 0321. 2 Supported in part by a grant from the National Science Foundation.DCB-8502215. (Received May 14, 1985; Accepted September 12, 1985)
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