Effect of heated naringenin on immunomodulatory properties and cellular antioxidant activity |
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Authors: | Mouna Maatouk Dorra Elgueder Nadia Mustapha Hind Chaaban Imen Mokdad Bzéouich Irina Loannou Soumaya Kilani Mohamed Ghoul Kamel Ghedira Leila Chekir-Ghedira |
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Institution: | 1.Unité des Substances Naturells Bioactives et Biotechnologie, Faculté de Pharmacie de Monastir,Université de Monastir,Monastir,Tunisia;2.Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir,Université de Monastir,Monastir,Tunisia;3.Laboratoire d’ingénierie des Biomolécules, ENSAIA-INPL,Université de Lorraine,Vandoeuvre-lès-Nancy,France |
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Abstract: | Naringenin is one of the most popular flavonoids derived from citrus. It has been reported to be an effective anti-inflammatory compound. Citrus fruit may be used raw, cooked, stewed, or boiled. The present study was conducted to investigate the effect of thermal processes on naringenin in its immunomodulatory and cellular antioxidant activities. The effects of flavonoids on B and T cell proliferation were assessed on splenocytes stimulated or not with mitogens. However, their effects on cytotoxic T lymphocyte (CTL) and natural killer (NK) activities were assessed in splenocytes co-incubated with target cells. The amount of nitric oxide production and the lysosomal enzyme activity were evaluated in vitro on mouse peritoneal macrophages. Cellular antioxidant activity in splenocytes and macrophages was determined by measuring the fluorescence of the dichlorofluorescin (DCF). Our findings revealed that naringenin induces B cell proliferation and enhances NK activity. The highest concentration of native naringenin exhibits a significant proliferation of T cells, induces CTL activity, and inhibits cellular oxidation in macrophages. Conversely, it was observed that when heat-processed, naringenin improves the cellular antioxidant activity in splenocytes, increases the cytotoxic activity of NK cells, and suppresses the cytotoxicity of T cells. However, heat treatment maintains the anti-inflammatory potency of naringenin. |
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