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Transference of a crystal protein gene from Bacillus thuringiensis and its expression in Bradyrhizobium sp. cells |
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| Authors: | da Costa Lima Paulo Sarmanho Lemos Manoel Victor Franco Lemos Eliana G Macedo Alves Lúcia Maria Carareto |
| Institution: | (1) EMBRAPA/CPAMN, Av. Duque de Caxias, 5650, 64056-220 Teresina, Brasil;(2) Depto. de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP, Via Prof. Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, SP, Brasil;(3) Depto. de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14870-000 Jaboticabal, SP, Brasil |
| Abstract: | The plasmid pHT409 that harbours the cryIA(a) gene for the production of a  -endotoxin (crystal protein) from Bacillus thuringiensis was transferred into Bradyrhizobium sp. A conjugal transfer system aiming to introduce the plasmid into the Bradyrhizobium sp. host from colonies of an Escherichia coli donor strain (DH5 ::pHT409) has been developed. As a result exconjugants were obtained in which the transferred plasmid has been detected by both microbiological and electrophoresis techniques. The cryIA(a) gene when inside the new host had a low expression level which was detected by immunoblotting. |
| Keywords: | Bacillus thuringiensis Bradyrhizobium sp cryIA(a) conjugation crystal protein plasmid stability |
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