Localization in situ of c-myc mRNA and c-myc protein in adult mouse testis |
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Authors: | Takehiko Koji Shinichi Izumi Masashi Tanno Tetsuya Moriuchi and Paul K Nakane |
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Institution: | (1) Department of Cell Biology, Tokai University School of Medicine, Bohseidai, 259-11 Isehara, Japan |
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Abstract: | Summary It has been suggested that c-myc, one of the proto-oncogenes, plays a role in normal somatic cell proliferation and differentiation. To define whether c-myc is only expressed during somatic cell division or is also expressed during meiotic cell division, the production of c-myc mRNA and protein were investigated in the mouse testis by usingin situ hybridization with non-radioactive DNA probes and enzyme immunohistochemistry respectively. Forin situ hybridization, T-T dimerized DNA probes were used and DNAs hybridizedin situ were detected immunohistochemically using specific antibody against T-T dimer. The results indicate that c-myc mRNA and protein are expressed in a cell-cycle-dependent manner only in spermatogonia and not in spermatocytes and spermatids. |
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