Pardaxin-induced apoptosis enhances antitumor activity in HeLa cells |
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Authors: | Hsu Jung-Chieh Lin Li-Ching Tzen Jason T C Chen Jyh-Yih |
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Affiliation: | a Graduate Institute of Biotechnology, National Chung-Hsing University, 250 Kuo-Kuang Rd., Taichung 402, Taiwan b Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, Ilan 262, Taiwan |
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Abstract: | Pardaxin, a pore-forming antimicrobial peptide that encodes 33 amino acids was isolated from the Red Sea Moses sole, Pardachirus mamoratus. In this study, we investigated its antitumor activity in human fibrosarcoma (HT-1080) cells and epithelial carcinoma (HeLa) cells. In vitro results showed that the synthetic pardaxin peptide had antitumor activity in these two types of cancer cells and that 15 μg/ml pardaxin did not lyse human red blood cells. Moreover, this synthetic pardaxin inhibited the proliferation of HT1080 cells in a dose-dependent manner and induced programmed cell death in HeLa cells. DNA fragmentation and increases in the subG1 phase and caspase 8 activities suggest that pardaxin caused HeLa cell death by inducing apoptosis, but had a different mechanism in HT1080 cells. |
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Keywords: | Antimicrobial peptides (AMPs) Pardaxin Cytotoxicity Pores |
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