INHIBITION OF MALIGNANT CELL PROLIFERATION BY CULTURE MEDIA CONDITIONED BY CARDIAC OR SKELETAL MUSCLE |
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| Authors: | TOVA ZINMAN SAMUEL SALZBERG ZVI MALIK ASHER SHAINBERG |
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| Affiliation: | 1. The Otto Meyerhoff Drug Receptor Center, Ramat Gan, 52900, Israel;2. Department of Life Sciences, Bar-Ilan University, Ramat Gan, 52900, Israel;1. School of computer science and technology, Taiyuan University of Science and Technology, China;2. State Key Laboratory of Intelligent Control and Management of Complex System, Institute of Automation, Chinese Academy of Sciences, China;3. University of Technology Sydney, Australia;1. School of Materials Science and Engineering, University of Science and Technology of China, Shenyang 110016, PR China;2. Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, PR China;3. Liaoning Shenyang Soil and Atmosphere Material Corrosion National Observation and Research Station, Shenyang 110016, PR China;1. Research and Extension Center for Irrigation and Agroclimatology (CITRA) and Research Program on Adaptation of Agriculture to Climate Change (A2C2), Faculty of Agricultural Sciences, Universidad de Talca, Campus Talca, Chile;2. Instituto de Investigaciones Agropecuarias (INIA) Intihuasi, Colina San Joaquín s/n, La Serena, Chile;3. Department of Agricultural Sciences, Universidad Católica del Maule, Curicó, Chile;4. C. Abud & Cia., Departamento de investigación, desarrollo e innovación. Curicó, Chile;1. Centro de Investigación en Ingenierías y Ciencias Aplicadas, Universidad Autónoma del Estado de Morelos, Av.Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos, Mexico;2. Instituto de Energías Renovables, Universidad Nacional Autónoma de México, Temixco, Morelos 62580, Mexico;3. Department of Materials Science & Engineering, University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75252, USA |
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| Abstract: | The present work is an attempt to explain the high resistance of muscles to cancer development. We used primary cultures of rat skeletal and cardiac muscle, and examined the effect of the supernatant of these cultures (conditioned medium; CM) on proliferation of cancer cells. The results demonstrated that CM inhibited the proliferation of several types of malignant cells by more than 50%, without a significant inhibition on normal cells. Cell cycle analysis revealed that CM increased the number of cells in S and G2 phases, suggesting a cytostatic effect of CM. For defining the biological properties of the factor(s) which are present in the CM, skeletal muscle cultures were grown in chemically defined medium (serum free medium). The concentrated sample was applied to a Sephadex G-50 column and three fractions were obtained. Only one fraction showed inhibitory activity. Four protein bands were observed in this fraction, as revealed by SDS-PAGE. We suggest that some, or all of these proteins are responsible for inhibition of tumor cell replication. |
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| Keywords: | muscle cultures anti tumor factor conditioned medium |
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