| 国产“水山姜”的分类学研究—来自核糖体DNA ITS区序列的证据 |
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| 引用本文： | 赵志礼,周开亚,董辉,徐珞珊. 国产“水山姜”的分类学研究—来自核糖体DNA ITS区序列的证据[J]. 云南植物研究, 2001, 23(4): 439-443 |
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| 作者姓名： | 赵志礼 周开亚 董辉 徐珞珊 |
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| 作者单位： | 1. 中国药科大学生药学研究室,江苏南京,210038
2. 南京师范大学遗传资源研究所,江苏,南京,210097 |
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| 基金项目： | 国家自然科学基金资助项目(39600010)及江苏省资源生物技术重点实验室资助项目 |
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| 摘 要： | 用直接测序法对国产黑果山姜Alpinia nigra(Gaertn.)Burtt以及“水山姜Alpinia aquatica (Koen.)Rose”。的核糖体DNA中的内转录间隔区（ITS）序列进行了测定,结果显示两者序列完全一致;ITS1长度为178bp,ITS2长度为232bp,5.8S编码区长度为164bp,GC含量为56.9%,形态学特征结合DNA分子证据,认为《中国植物志》记载的水山姜实为黑果山姜。
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| 关 键 词： | 核糖体DNA ITS序列 黑果山姜 水山姜 分类学研究 |
| 文章编号： | 0253-2700(2001)04-0439-05 |
| 修稿时间： | 2000-08-09 |
Studies on Systematics of "Alpinia aquatica " from China:Evidence from ITS Sequences of Nuclear Ribosomal DNA |
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| ZHAO Zhi-Li ,ZHOU Kai-Ya ,DONG Hui ,XU Luo-Shan. Studies on Systematics of "Alpinia aquatica " from China:Evidence from ITS Sequences of Nuclear Ribosomal DNA[J]. Acta Botanica Yunnanica, 2001, 23(4): 439-443 |
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| Authors: | ZHAO Zhi-Li ZHOU Kai-Ya DONG Hui XU Luo-Shan |
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| Affiliation: | ZHAO Zhi-Li 1,ZHOU Kai-Ya 2,DONG Hui 1,XU Luo-Shan 1 |
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| Abstract: | Nuclear ribosomal DNA internal transcribed spacer (ITS) regions of Alpinia nigra (Gaertn.) Burtt and "Alpinia aquatica (Koen.) Rosc." from China were directly sequenced using an automated sequencer. The result showed that both sequences were identical. The size is 178 bp in ITS 1, 232 bp in ITS 2 and 164 bp in 5.8S coding region, G C content being 56.9%. According to the morphological and DNA characters, it was suggested that "Alpinia aquatica (Koen.) Rosc." recorded in Flora of China should be Alpinia nigra (Gaertn.) Burtt. |
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| Keywords: | nrDNA ITS sequence Alpinia nigra Alpinia aquatica Systematics |
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