Thymus-expressed chemokine promotes survival of PC12 cells via PI3K pathway |
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Authors: | Cao Xia Ma Jun Wu Guodong Zhang Caijun Wang Ling Dai Shuying Xu Wei |
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Institution: | Department of Immunology and Microbiology, Faculty of Basic Medical Sciences, Kunming Medical College, Kunming, Yunnan, PR China. |
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Abstract: | We reported previously that CCR9 was neuroprotective in the mouse hippocampal neurons. This study was aimed to investigate if thymus-expressed chemokine (TECK)/CCL25 could promote survival of PC12 cells though its receptor CCR9. pEGFP-N1/CCR9 recombinant was constructed and transfected into PC12 cells. Along with this, 50 nM NGF was used to induce PC12 cells to differentiate into sympathetic-like neurons. We show here that under serum-free conditions and within a concentration range (50-200 nM), TECK rescued pEGFP-N1/CCR9 transfected PC12 cells from undergoing apoptosis in serum-free medium; however, it did not exert a similar effect on the cells in the control. On the other hand, the PC12 cells succumbed to a higher concentration of TECK (≥ 300 nM). Bim expression was up-regulated in PC12 cells cultured in serum-free medium in the absence of factors or with anti-TECK+TECK; however, it was not up-regulated in TECK-treated PC12 cells. p-Akt was detected at 15 min which lasted for at least 60 min when PC12 cells were cultured in serum-free medium with TECK. Additionally, it was shown that such an effect was effectively blocked by PI3K inhibitor, Wortmannin. These data suggest that TECK promotes survival of serum-deprived PC12 cells through its receptor, CCR9, most likely via the PI3K/Akt signaling pathway. |
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Keywords: | TECK thymus-expressed chemokine CCR9 chemokine receptor 9 CCL25 chemokine ligand 25 NGF nerve growth factor GFAP glial fibrillar acidic protein PBS phosphate buffered saline RT-PCR real time-polymerase chain reaction MTT 3-(4 5)-dimethylthiahiazo(-z-y1)-3 5-di-phenytetrazoliumromide FBS fetal bovine serum ELISA enzyme-linked immunosorbent assay |
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