Studies of protoplast culture and plant regeneration from commercial and rapid-cyclingBrassica species |
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| Authors: | Peter T. Loudon Robin S. Nelson David S. Ingram |
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| Affiliation: | (1) University of Cambridge, Department of Botany, The Botany School, Downing Street, CB2 3EA Cambridge, UK;(2) NERC Institute of Virology, Mansfield Road, OX1 3SR Oxford, UK;(3) The Nickerson Seed Company Ltd., Cambridge Science Park, Milton Road, CB4 4GZ Cambridge, UK |
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| Abstract: | Protoplasts were isolated from aseptic shoot cultures of commercial cultivars ofBrassica napus, B. oleracea andB. campestris, and from the six  rapid-cycling brassica species . Of the rapid-cycling species, onlyB. napus responded well to the culture conditions used; 2% of protoplasts formed calli and up to 5% of calli regenerated shoots. Regeneration was also achieved from commercial cultivars ofB. napus andB. oleracea. For these two species the plating density, time of dilution with fresh medium and the composition of the shoot-inducing medium were all found to have an important influence on the efficiency of plant regeneration. Both responded better to maltose than to sucrose-based media. Under the optimum conditionsB. napus showed a plating efficiency of 7.8% and shooting efficiency of 17%; forB. oleracea the figures were 2% and 56%, respectively.Abbreviations BAP 6-benzylaminopurine - NAA  -naphthaleneacetic acid - 2,4-D 2,4-dichlorophenoxyacetic acid |
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| Keywords: | Brassica napus B. oleracea rapid-cycling brassica populations protoplasts regeneration maltose |
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