Human Papillomavirus Detection In Cervical Cells By In Situ Hybridization With Biotinylated Probes |
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Authors: | Y. CHARDONNET,F. BÉ JUI-THIVOLET,I. GUÉ RIN-REVERCHON |
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Affiliation: | INSERM U346, Affiliée CNRS, Pav. R, H?pital E. Herriot, Lyon, France. |
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Abstract: | We have investigated the applicability of human papillomavirus (HPV) DNA detection by in situ hybridization with biotinylated probes in epithelial cells obtained from the cervix using a cotton tip swab. We describe a simple procedure for obtaining homogeneous cell samples and good preservation of cellular structure. This is achieved by pretreatment of cells with L-cysteine before hybridization. Separate denaturation of cellular DNA and probe DNA is also necessary for satisfactory results. Both benign HPV DNA 6/11 and potentially oncogenic HPV DNA 16/18 could be identified in our series. In situ hybridization on cervical scrapes is a rapid, simple and very specific method for detecting patients infected with oncogenic HPV types. |
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Keywords: | cervical scrapes in situ hybridization HPV biotinylated probes |
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