Two motifs essential for nuclear import of the hnRNP A1 nucleocytoplasmic shuttling sequence M9 core |
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Authors: | Iijima Megumi Suzuki Maiko Tanabe Ayako Nishimura Akira Yamada Michiyuki |
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Institution: | Graduate School of Integrated Science, Yokohama City University, 22-2, Seto, Kanazawa-ku, Yokohama 236-0027, Japan. |
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Abstract: | Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 regulates mRNA genesis. It shuttles between the nucleus and cytoplasm. Its shuttling signal is a 38-residue sequence M9. We studied the nuclear import and export of M9 by mutational analysis. Heterokaryon assay indicated that the 19-residue sequence SNFGPMKGGNFGGRSSGPY (M9 core) is necessary and sufficient for shuttling. Moreover, M9 core mutation revealed that in addition to the hitherto characterized N-terminal motif SNFGPMK, the C-terminal motif PY is crucial for nuclear import as well as for binding to transportin. Key residues of the motifs are conserved in the shuttling signals of hnRNP D and JKTBP. |
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Keywords: | hnRNP A1 Nucleocytoplasmic transport Transportin NLS NES |
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