| 锌离子存在下EGCG对前列腺癌细胞生长的影响 |
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| 引用本文： | 张岚翠,沈生荣,孙世利,杨军国,何国庆,于海宁.锌离子存在下EGCG对前列腺癌细胞生长的影响[J].分子细胞生物学报,2008,41(6). |
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| 作者姓名： | 张岚翠 沈生荣 孙世利 杨军国 何国庆 于海宁 |
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| 作者单位： | [1]浙江大学茶学系,杭州310029 [2]浙江大学生物系统工程与食品科学学院,杭州310029 [3]浙江工业大学药学院,杭州310032 |
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| 基金项目： | 国家自然科学基金资助项目
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| 摘 要： | 本文研究了锌离子存在下EGCG对前列腺癌细胞PC-3生长的影响．研究发现Zn^2＋可以增强EGCG抗癌活性，Zn^2＋存在下。EGCG处理后前列腺癌细胞PC-3克隆形成率显著下降。以RT—PCR、免疫组化方法研究Zn^2＋、EGCG对67kD层粘连蛋白受体（67kD Laminin Receptor，67LR）表达调控，结果表明Znn可通过上调67LR的表达，为EGCG提供更多作用的靶位点，增强EGCG对前列腺癌细胞PC-3的毒性作用。MMP-9是肿瘤侵袭转移过程中关键的基质金属蛋白酶。MMP-9活性与癌细胞的转移潜能密切相关。本文研究发现Zn^2＋、EGCG处理可通过抑制MMP-9活性，降低前列腺癌细胞PC-3的迁移率．其中80umol／LEGCG＋80umol／L Zn^2＋处理24h后显著抑制了PC-3细胞的迁移率。
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| 关 键 词： | 锌离子 |
GROWTH INHIBITION OF PROSTATE CANCER CELLS BY EPIGALLOCATECHIN-3-GALLATE IN THE PRESENCE OF Zn~(2+)IN VITRO |
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| ZHANG Lan Cui SHEN Sheng Rong SUN Shi Li YANG Jun Guo HE Guo Qing YU Hai Ning.GROWTH INHIBITION OF PROSTATE CANCER CELLS BY EPIGALLOCATECHIN-3-GALLATE IN THE PRESENCE OF Zn~(2+)IN VITRO[J].Journal of Molecular Cell Biology,2008,41(6). |
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| Authors: | ZHANG Lan Cui SHEN Sheng Rong SUN Shi Li YANG Jun Guo HE Guo Qing YU Hai Ning |
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| Institution: | ~1 Department of Tea Sciences;Zhejiang University;Hangzhou 310029;~2 Department of Food Science and Nutrition;~3 College of Pharmaceutical Sciences;Zhejiang University of Technology;Hangzhou 310032 |
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| Abstract: | In this paper,the cytotoxicity of EGCG against PC-3 prostate cancer cells and its molecular mechanism in the presence and absence of Zn~(2+)in vitro were investigated.The results showed that both EGCG and Zn~(2+)suppressed clonegenecity of PC-3 cells,and the suppression ef- fect was enhanced in the coexist system of EGCG and Zn~(2+).MMP-9 is thought to play a significant role in cancer cell migration and invasion.In the present paper,the results showed that EGCG suppressed the activity of MMP-9 in PC-3 cell... |
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| Keywords: | EGCG PC-3 |
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