Characteristics of the epitope of leukotriene B4 recognized by a highly specific mouse monoclonal antibody |
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Authors: | J Y Lee T Chernov E J Goetzl |
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Institution: | Howard Hughes Medical Institute and the Department of Medicine, University of California Medical Center, San Francisco, California 94143, USA |
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Abstract: | Mouse monoclonal IgG2b antibodies to leukotriene B4 bind 3H]leukotriene B4 with an affinity one-thirtieth to one-third that of different rabbit antibodies to leukotriene B4. The concentrations of related ligands required to inhibit by 50% the binding of 3H]leukotriene B4 define cross-reactivities of approximately 100% for carboxyl-derivatives of leukotriene B4, 10% for 12(S)-leukotriene B4 and 8 cis-leukotriene B4, which were not distinguished from leukotriene B4 by polyclonal antibodies, 3-5% for the two isomers of 6 trans-leukotriene B4, 5% for 20-OH-leukotriene B4 and 20-COOH-leukotriene B4, and less than 1% for other leukotrienes, mono-hydroxy-eicosatetraenoic acids, and the two leukotriene B4-like isomers of 8, 15-di-hydroxy-eicosatetraenoic acid. Thus the monoclonal combining site is highly specific for the di-hydroxy-triene portion of leukotriene B4. |
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Keywords: | PMN polymorphonuclear BGG bovine gamma globulin HSA human serum albumin EDC 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride HPLC high performance liquid chromatography 8(R) 15(S)-LT 8(S) 15(S)-LT TETA triethylenetetramine tetrahydrochloride HAT hypoxanthine aminopterin and thymidine PEG polyethylene glycol |
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