Activation of cGMP phosphodiesterase by purified green rod pigment from frog retina |
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Authors: | T Shinozawa M Makino-Tasaka T Suzuki |
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Affiliation: | 1. Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606 Japan;2. Department of Pharmacology, Hyogo College of Medicine, Nishinomiya, Hyogo 663, Japan |
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Abstract: | Activation of cGMP phosphodiesterase(PDE) of frog rod outer segments (ROS) by purified green rod pigment (GRP) was analyzed. GRP activated PDE in a similar manner to purified rhodopsin. This activation required illumination of the pigment and presence of GTP. |
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Keywords: | Frog retina Green rod pigment Rhodopsin cGMP Phosphodiesterase Visual excitation PDE, phosphodiesterase ROS, rod outer segments GRP, green rod pigment SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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