Somatic embryogenesis and plant regeneration of squash Cucurbita pepo L cv. YC 60 |
| |
Authors: | Paula P. Chee |
| |
Affiliation: | (1) Molecular Biology Division, The Upjohn Co., 301 Henrietta St., 49007 Kalamazoo, MI, USA |
| |
Abstract: | Plant regeneration from tissue cultures of summer squash, Cucurbita pepo L., cv. YC60, has been observed. Somatic embryos organized from shoot apex derived callus cultured on Murashige and Skoog (MS) medium supplemented with 1.2 mg/l 2,4,5-trichlorophenoxyacetic acid, 0.8 mg/l benzylaminopurine, and 0.1 mg/l kinetin. Embryos developed into plantlets by transfer of immature somatic embryos to MS medium with 0.05 mg/l NAA and 0.05 mg/l kinetin. Regenerated plants appeared morphologically normal and set fruits with seeds which could germinate normally.Abbreviation BAP 6-benzylaminopurine - 2,4-D 2, 4dichlorophenoxyacetic acid - IAA indole-3-acetic acid - KN kinetin - NAA -naphthyleneacetic acid - MS Murashige and Skoog - 2,4,5-T 2, 4,5-trichlorophenoxyacetic acid |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|